Carotenoids and Tocopherol Profiling in Fleshy Fruits

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Methods Mol Biol. 2024;2798:141-151. doi: 10.1007/978-1-0716-3826-2_9.

ABSTRACT

Carotenoids and tocopherols are among the most powerful lipophilic antioxidants accumulated in fruit and vegetable crops. This chapter describes a method for the separation and quantification of carotenoids/chlorophylls and tocopherols based on microextraction followed by reverse- and normal-phase HPLC, respectively. Using this method, high-throughput, accurate analysis of these compounds can be performed in leaf and fruit samples.

PMID:38587740 | DOI:10.1007/978-1-0716-3826-2_9